3SS4
Crystal structure of mouse Glutaminase C, phosphate-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-08 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97910 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.720, 136.280, 176.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.908 - 2.850 |
R-factor | 0.2291 |
Rwork | 0.227 |
R-free | 0.26960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3czd |
RMSD bond length | 0.013 |
RMSD bond angle | 1.021 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.500 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.152 | |
Number of reflections | 51943 | |
<I/σ(I)> | 5.1 | 1.7 |
Completeness [%] | 94.2 | 91 |
Redundancy | 3.5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 17% PEG3350, 0.2M NaCl, 0.1M Bis-TRIS, VAPOR DIFFUSION, SITTING DROP, temperature 291K, pH 6.5 |