3RYH
GMPCPP-Tubulin: RB3 Stathmin-like domain complex
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 65.270, 128.210, 250.430 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 42.130 - 2.800 |
| R因子 | 0.1676 |
| Rwork | 0.165 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hkb |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.190 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.8.0) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 45.000 | 2.870 |
| 分解能 [Å] (高) | 2.800 | 2.800 |
| 独立反射数 | 51713 | |
| <I/σ(I)> | 10.2 | 2 |
| 完全性 [%] | 99.3 | 89 |
| 冗長性 | 4 | 3.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | PEG, LISO4, PIPES BUFFER, pH 6.80, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
