3RIY

Sirt5 is an NAD-dependent protein lysine demalonylase and desuccinylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	52.691, 69.417, 156.321
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.550
R-factor	0.1461
Rwork	0.144
R-free	0.19130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rig
RMSD bond length	0.023
RMSD bond angle	2.027
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.066	0.046	0.398
Number of reflections	83831
<I/σ(I)>	13.1
Completeness [%]	99.7	98.4	98.5
Redundancy	7.1	6.7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	16% PEG 4000, 6% Glycerol, vapor diffusion, hanging drop, temperature 298K