3R8F
Replication initiator DnaA bound to AMPPCP and single-stranded DNA
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-28 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 99.797, 114.219, 201.266 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 43.487 - 3.366 |
R因子 | 0.2499 |
Rwork | 0.249 |
R-free | 0.26820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hcb |
結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
結合角の平均二乗偏差(RMSD) [度] | 0.864 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 50.000 | 3.470 |
分解能 [Å] (高) | 3.350 | 3.350 |
独立反射数 | 32862 | |
<I/σ(I)> | 13.5 | 2 |
完全性 [%] | 98.9 | 99.6 |
冗長性 | 3.5 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 15-35 mM sodium cacodylate, pH 6.5, 26% 1,2-propanediol, 1-2% PEG2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 291K |