3QRF

Structure of a domain-swapped FOXP3 dimer

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
格子定数 [Å]	83.667, 131.234, 68.665
格子定数 [度]	90.00, 89.95, 90.00

精密化法

残基	50.000 - 2.800
Rwork	0.248
R-free	0.28280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a02 - NFAT + DNA only
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.930
分解能 [Å] (高)	2.800	2.800
Rmerge_l_obs		1.021
独立反射数	35150
<I/σ(I)>		4.15
完全性 [%]	96.2	99.3

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.63	291	5 mM HEPES, pH 7.63, 2 mM dithiothreitol (DTT), 0.5 mM EDTA, and 150 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K