3QKR
Mre11 Rad50 binding domain bound to Rad50
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 |
| Unit cell lengths | 116.041, 116.041, 109.853 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.400 |
| R-factor | 0.21432 |
| Rwork | 0.211 |
| R-free | 0.27444 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ii8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.488 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.109 | 0.335 |
| Number of reflections | 10679 | |
| <I/σ(I)> | 14.2 | 2 |
| Completeness [%] | 91.5 | 56.39 |
| Redundancy | 6.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris, 200-300 mM lithium sulfate, 12-13% PEG3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
