3PYC

Crystal structure of human SMURF1 C2 domain

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
格子定数 [Å]	30.959, 47.048, 91.266
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	32.760 - 1.960
R因子	0.20582
Rwork	0.204
R-free	0.24115
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.018
結合角の平均二乗偏差(RMSD) [度]	1.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall
分解能 [Å] (低)	50.000
分解能 [Å] (高)	1.960
独立反射数	10113
完全性 [%]	99.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M HEPES (pH 6.5), 2M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K