3PTA
Crystal structure of human DNMT1(646-1600) in complex with DNA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Detector technology | CCD |
| Collection date | 2009-03-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2827 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 162.800, 87.900, 113.900 |
| 格子定数 [度] | 90.00, 97.20, 90.00 |
精密化法
| 残基 | 30.000 - 3.600 |
| R因子 | 0.257 |
| Rwork | 0.257 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 3.730 |
| 分解能 [Å] (高) | 3.600 | 3.600 |
| 独立反射数 | 19075 | |
| <I/σ(I)> | 16.6 | 1.8 |
| 完全性 [%] | 99.0 | 94.8 |
| 冗長性 | 7.2 | 4.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 6% PEG 20,000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
