3P87
Structure of human PCNA bound to RNASEH2B PIP box peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.982 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.108, 81.788, 116.558 |
| Unit cell angles | 90.00, 91.47, 90.00 |
Refinement procedure
| Resolution | 47.450 - 2.990 |
| R-factor | 0.2241 |
| Rwork | 0.223 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1axc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.090 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 3.090 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.126 | 0.852 |
| Number of reflections | 31807 | |
| <I/σ(I)> | 8 | 1.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293.15 | 3M NaCl, 0.1M citrate, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
