3OWT

Crystal structure of S. cerevisiae RAP1-Sir3 complex

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	H 3 2
格子定数 [Å]	89.831, 89.831, 211.791
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	38.258 - 2.000
R因子	0.2151
Rwork	0.210
R-free	0.26040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cz6
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.018
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Quality characteristics

	Overall	Inner shell	Outer shell
分解能 [Å] (低)	50.000	50.000	1.850
分解能 [Å] (高)	1.780	3.860	1.790
Rmerge_l_obs	0.064	0.061	0.313
独立反射数	27940
完全性 [%]	89.3	99.1	33.4
冗長性	9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	4.8	277	100 mM sodium citrate pH 4.8, 30% PEG4K, 200 mM ammonium acetate, and 10 mM DTT , VAPOR DIFFUSION, HANGING DROP, temperature 277K