3ON3

The crystal structure of keto/oxoacid ferredoxin oxidoreductase, gamma subunit from Geobacter sulfurreducens PCA

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97926
Spacegroup name	P 3 2 1
格子定数 [Å]	91.332, 91.332, 74.191
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	39.548 - 2.193
R因子	0.1988
Rwork	0.196
R-free	0.26010
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.022
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXD
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	40.000	2.240
分解能 [Å] (高)	2.193	2.200
Rmerge_l_obs	0.155	0.827
独立反射数	18254
<I/σ(I)>	20.6	1.5
完全性 [%]	98.0	85.7
冗長性	9.2	7.8

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.2M sodium chloride, 0.1M Sodium cacodylate, 2M Ammonium sulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K