3O7A
Crystal structure of PHF13 in complex with H3K4me3
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 21.232, 44.334, 60.330 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 35.730 - 1.670 |
| R因子 | 0.1851 |
| Rwork | 0.183 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o70 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.020 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.586 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 50.000 |
| 分解能 [Å] (高) | 1.660 |
| Rmerge_l_obs | 0.032 |
| 独立反射数 | 6772 |
| 冗長性 | 7.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1M Tris-HCl pH 8.5; 1.5M Na-Citrate, PEG400 6%, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
