3NMT
Crystal structure of pyrabactin bound abscisic acid receptor PYL2 mutant A93F in complex with type 2C protein phosphatase HAB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.020, 66.877, 144.441 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.850 - 2.560 |
| R-factor | 0.22707 |
| Rwork | 0.224 |
| R-free | 0.26577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kb3 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.395 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.550 |
| Rmerge | 0.164 |
| Number of reflections | 19661 |
| <I/σ(I)> | 15.4 |
| Completeness [%] | 80.2 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M ammonium sulphate, 0.1M Tris, 10% ethyl glycol, 23% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
