3NMP
Crystal structure of the abscisic receptor PYL2 mutant A93F in complex with pyrabactin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.084, 105.571, 182.901 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.390 - 2.100 |
R-factor | 0.23583 |
Rwork | 0.234 |
R-free | 0.26634 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nmh |
RMSD bond length | 0.020 |
RMSD bond angle | 1.555 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.093 |
Number of reflections | 32811 |
<I/σ(I)> | 27.6 |
Completeness [%] | 87.0 |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 2M ammonium acetate, 22% PEG 3350, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |