3MYU

Mycoplasma genitalium MG289

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-03
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
格子定数 [Å]	49.279, 90.366, 175.363
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	45.180 - 1.950
R因子	0.2222
Rwork	0.221
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eki
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	0.938
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((AUTOMR: 1.6.1_348))
Refinement software	PHENIX ((phenix.refine: 1.6.1_348))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	45.180	2.020
分解能 [Å] (高)	1.950	1.950
Rmerge_l_obs	0.076	0.560
独立反射数	52800
<I/σ(I)>	22	2.7
完全性 [%]	90.9	90
冗長性	7.2	6.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		4.6	298	0.2 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE TRIHYDRATE pH 4.6 and 15% PEG 4000 AND HAMPTON SILVER BULLET BIO CONDITION 49, VAPOR DIFFUSION, HANGING DROP, temperature 298K