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3MD3

Crystal Structure of the First Two RRM Domains of Yeast Poly(U) Binding Protein (Pub1)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RUH3R
Detector technologyIMAGE PLATE
Collection date2009-05-22
DetectorMAR scanner 345 mm plate
Spacegroup nameP 61 2 2
Unit cell lengths57.615, 57.615, 237.082
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution49.900 - 2.700
R-factor0.218
Rwork0.216
R-free0.26800
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle0.939
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareMOLREP
Refinement softwareREFMAC
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]50.00050.0002.800
High resolution limit [Å]2.7005.8102.700
Rmerge0.1170.0680.324
Number of reflections7092
<I/σ(I)>7.5
Completeness [%]99.395.799.7
Redundancy65.26.2
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP52832.0M ammonium sulfate, 0.1M sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K

217705

數據於2024-03-27公開中

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