3MD3
Crystal Structure of the First Two RRM Domains of Yeast Poly(U) Binding Protein (Pub1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Detector technology | IMAGE PLATE |
Collection date | 2009-05-22 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 57.615, 57.615, 237.082 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.900 - 2.700 |
R-factor | 0.218 |
Rwork | 0.216 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.939 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.117 | 0.068 | 0.324 |
Number of reflections | 7092 | ||
<I/σ(I)> | 7.5 | ||
Completeness [%] | 99.3 | 95.7 | 99.7 |
Redundancy | 6 | 5.2 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 283 | 2.0M ammonium sulfate, 0.1M sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K |