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3MD1

Crystal Structure of the Second RRM Domain of Yeast Poly(U)-Binding Protein (Pub1)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsBSRF BEAMLINE 3W1A
Synchrotron siteBSRF
Beamline3W1A
Temperature [K]100
Detector technologyCCD
Collection date2009-01-12
DetectorMAR CCD 130 mm
Wavelength(s)1.000
Spacegroup nameH 3
Unit cell lengths72.661, 72.661, 81.989
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution49.920 - 1.600
R-factor0.19929
Rwork0.198
R-free0.22051
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1cvj
RMSD bond length0.009
RMSD bond angle1.105
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASES
Refinement softwareREFMAC (5.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.00050.000
High resolution limit [Å]1.6001.600
Rmerge0.340
Number of reflections20050
Redundancy5.75.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP4.528325% (w/v) polyethylene glycol 4000 (PEG4000), 0.1 M sodium acetate , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K

217705

數據於2024-03-27公開中

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