3MD1
Crystal Structure of the Second RRM Domain of Yeast Poly(U)-Binding Protein (Pub1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-12 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.000 |
Spacegroup name | H 3 |
Unit cell lengths | 72.661, 72.661, 81.989 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.920 - 1.600 |
R-factor | 0.19929 |
Rwork | 0.198 |
R-free | 0.22051 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cvj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.105 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.340 | |
Number of reflections | 20050 | |
Redundancy | 5.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 283 | 25% (w/v) polyethylene glycol 4000 (PEG4000), 0.1 M sodium acetate , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |