3M1R

The crystal structure of formimidoylglutamase from Bacillus subtilis subsp. subtilis str. 168

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-19
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
格子定数 [Å]	142.038, 118.980, 123.340
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	32.474 - 2.199
R因子	0.1791
Rwork	0.176
R-free	0.23020
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.154
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXD
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	32.500	2.290
分解能 [Å] (高)	2.250	2.250
Rmerge_l_obs	0.100	0.726
独立反射数	102844
<I/σ(I)>	30.6	3.1
完全性 [%]	99.6	99.9
冗長性	7.3	7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.2M MgCl2, 0.1M Sodium cacodylate, 50% (v/v) PEG200, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K