3LXF
Crystal Structure of [2Fe-2S] Ferredoxin Arx from Novosphingobium aromaticivorans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-07-21 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.554, 78.788, 89.200 |
Unit cell angles | 90.00, 125.04, 90.00 |
Refinement procedure
Resolution | 28.160 - 2.300 |
R-factor | 0.20184 |
Rwork | 0.200 |
R-free | 0.23929 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.754 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.070 | 0.451 |
Number of reflections | 34610 | |
<I/σ(I)> | 22.8 | 2.5 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 4.1 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M HEPES, 1.4M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |