3LRA

Structural Basis for Assembling a Human Tripartite Complex Dlg1-MPP7-Mals3

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-30
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 62 2 2
格子定数 [Å]	153.190, 153.190, 58.903
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	38.300 - 2.950
R因子	0.255
Rwork	0.255
R-free	0.28400
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.2)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	38.300	3.060
分解能 [Å] (高)	2.950	2.950
Rmerge_l_obs	0.092	0.435
独立反射数	8045
<I/σ(I)>	25.7	2.1
完全性 [%]	95.0	77.7
冗長性	14.8	4.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7	293	10% PEG 8000, 0.2M MgCl2, 0.1M Guanidine HCl, 0.1M Tris-HCl, pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7	293	10% PEG 8000, 0.2M MgCl2, 0.1M Guanidine HCl, 0.1M Tris-HCl, pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K