3KL4
Recognition of a signal peptide by the signal recognition particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.883, 91.883, 133.263 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.420 - 3.500 |
R-factor | 0.301 |
Rwork | 0.301 |
R-free | 0.32200 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.619 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.420 | 3.690 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.081 | 0.762 |
Number of reflections | 7683 | |
<I/σ(I)> | 29.2 | 5.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 26.7 | 27.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 295 | 5-7 % PEG 4000, 100 mM Bis-Tris, 100 mM NaCl, 5-50 mM Mg(OAc)2, 2 % Polypropylene glycol P400. Crystals were obtained by seeding, pH 5.5, VAPOR DIFFUSION, temperature 295K |
1 | VAPOR DIFFUSION | 5.5 | 295 | 5-7 % PEG 4000, 100 mM Bis-Tris, 100 mM NaCl, 5-50 mM Mg(OAc)2, 2 % Polypropylene glycol P400. Crystals were obtained by seeding, pH 5.5, VAPOR DIFFUSION, temperature 295K |
1 | VAPOR DIFFUSION | 5.5 | 295 | 5-7 % PEG 4000, 100 mM Bis-Tris, 100 mM NaCl, 5-50 mM Mg(OAc)2, 2 % Polypropylene glycol P400. Crystals were obtained by seeding, pH 5.5, VAPOR DIFFUSION, temperature 295K |