3KJY
Crystal structure of reduced HOMO SAPIENS CLIC3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.080, 48.380, 59.760 |
| Unit cell angles | 69.68, 80.89, 74.60 |
Refinement procedure
| Resolution | 30.260 - 1.950 |
| R-factor | 0.2003 |
| Rwork | 0.198 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fy7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.021 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.4_161)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.260 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.036 | 0.406 |
| Number of reflections | 32724 | |
| <I/σ(I)> | 15.7 | 2.1 |
| Completeness [%] | 92.5 | 89.4 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Solution (15mg/ml protein in 20mm HEPES-NAOH, PH 7.5, 200mm NACl) plus 3 micro-l of reservoir solutionl (0.95M NH4SO4,0.225M LISO4, 0.1M TRIS-HCL PH 8.5), drop equilibrated against 1 ml reservoir solution , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, CRYO Reservoir + 20%V Ethylene glycol |
