3KJY
Crystal structure of reduced HOMO SAPIENS CLIC3
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 48.080, 48.380, 59.760 |
| 格子定数 [度] | 69.68, 80.89, 74.60 |
精密化法
| 残基 | 30.260 - 1.950 |
| R因子 | 0.2003 |
| Rwork | 0.198 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fy7 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.021 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.4_161)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.260 | 2.060 |
| 分解能 [Å] (高) | 1.950 | 1.950 |
| Rmerge_l_obs | 0.036 | 0.406 |
| 独立反射数 | 32724 | |
| <I/σ(I)> | 15.7 | 2.1 |
| 完全性 [%] | 92.5 | 89.4 |
| 冗長性 | 2.2 | 2.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Solution (15mg/ml protein in 20mm HEPES-NAOH, PH 7.5, 200mm NACl) plus 3 micro-l of reservoir solutionl (0.95M NH4SO4,0.225M LISO4, 0.1M TRIS-HCL PH 8.5), drop equilibrated against 1 ml reservoir solution , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, CRYO Reservoir + 20%V Ethylene glycol |
