3KCV
Structure of formate channel
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 |
| 格子定数 [Å] | 107.034, 107.034, 285.452 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 48.610 - 3.198 |
| R因子 | 0.1967 |
| Rwork | 0.193 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kcu |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.607 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 48.610 | 3.310 |
| 分解能 [Å] (高) | 3.198 | 3.198 |
| Rmerge_l_obs | 0.100 | 0.729 |
| 独立反射数 | 60335 | |
| <I/σ(I)> | 13.3 | 1.6 |
| 完全性 [%] | 99.6 | 99.9 |
| 冗長性 | 3.4 | 3.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M MOPS, pH 7.5, 36% (w/v) PEG 400, 200mM NaCl or sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
