3KAY
Crystal structure of abscisic acid receptor PYL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-16 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 |
Unit cell lengths | 126.764, 126.764, 61.407 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.570 - 2.400 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.387 |
Data reduction software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 22196 |
Completeness [%] | 72.0 |
Redundancy | 22.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | PEG 4000, pH 7.5, vapor diffusion, temperature 298K |