3JZQ

Human MDMX liganded with a 12mer peptide inhibitor (pDIQ)

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
格子定数 [Å]	44.925, 53.735, 87.294
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	34.500 - 1.800
Rwork	0.208
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dab
結合長の平均二乗偏差(RMSD) [Å]	0.016
結合角の平均二乗偏差(RMSD) [度]	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	1.860
分解能 [Å] (高)	1.800	1.800
Rmerge_l_obs	0.062	0.470
独立反射数	20193
<I/σ(I)>	27.5	3.7
完全性 [%]	99.7	99
冗長性	6.9	6.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.4	292	2.1 M ammonium sulfate, 10 mM Tris HCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 292K