3JCN
Structures of ribosome-bound initiation factor 2 reveal the mechanism of subunit association: Initiation Complex I
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
Method | Blot for 2-4 seconds before plunging |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2 |
Detector | GATAN K2 (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.6 |
Software | CTFFIND,EMAN,SPARX,SPIDER |
Symmetry | C1 |