3J81
CryoEM structure of a partial yeast 48S preinitiation complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | Blot for 2.5 seconds before plunging |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | FEI FALCON II (4k x 4k) |
| Number of Images | 1791 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4 |
| Software | REFMAC,UCSF Chimera,EMAN,RELION |
| Symmetry | C1 |
