3ISR
The Crystal Structure of a Putative Cysteine Protease from Cytophaga hutchinsonii to 1.9A
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 90.716, 115.240, 131.262 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 48.280 - 1.900 |
| R因子 | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22000 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.207 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (refmac_5.5.0102) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 1.970 |
| 分解能 [Å] (高) | 1.900 | 4.090 | 1.900 |
| Rmerge_l_obs | 0.073 | 0.049 | 0.689 |
| 独立反射数 | 108506 | ||
| <I/σ(I)> | 8.7 | ||
| 完全性 [%] | 99.5 | 97.2 | 100 |
| 冗長性 | 4.9 | 4.8 | 4.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 6.9 | 289 | 20% PEG 3350, 0.2M Sodium thiocyanate, pH 6.9, vapor diffusion, temperature 289K |
