3IFU
The Crystal Structure of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Leader Protease Nsp1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 1W2B |
Synchrotron site | BSRF |
Beamline | 1W2B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-03-05 |
Detector | MAR555 FLAT PANEL |
Wavelength(s) | 1.2821, 1.2827 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 51.000, 51.000, 154.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.420 |
R-factor | 0.231 |
Rwork | 0.223 |
R-free | 0.25700 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 2.070 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.420 | 2.420 |
Rmerge | 0.065 | 0.419 |
Number of reflections | 8279 | |
Completeness [%] | 99.0 | 95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100mM Tris-HCl, 25%(v/v) tert-butanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |