3IDD
Cofactor-Independent Phosphoglycerate Mutase from Thermoplasma acidophilum DSM 1728
Experimental procedure
実験手法 | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97924, 0.97948 |
Spacegroup name | P 21 21 2 |
格子定数 [Å] | 88.605, 136.744, 63.289 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 74.360 - 2.800 |
R因子 | 0.23439 |
Rwork | 0.230 |
R-free | 0.31106 |
Structure solution method | MAD |
結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
結合角の平均二乗偏差(RMSD) [度] | 1.610 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.5.0072) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 74.360 | 2.620 |
分解能 [Å] (高) | 2.600 | 2.600 |
Rmerge_l_obs | 0.098 | 0.591 |
独立反射数 | 22991 | |
<I/σ(I)> | 1.5 | |
完全性 [%] | 99.0 | 99 |
冗長性 | 3.7 | 2.6 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.20 M NaCl, 0.10 M phosphate citrate, pH 4.2, 10% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |