3HSI
Crystal structure of phosphatidylserine synthase Haemophilus influenzae Rd KW20
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | I 2 2 2 |
| 格子定数 [Å] | 102.555, 154.238, 214.420 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 2.200 |
| R因子 | 0.1923 |
| Rwork | 0.190 |
| R-free | 0.23608 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.409 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.220 |
| 分解能 [Å] (高) | 2.200 | 2.200 |
| Rmerge_l_obs | 0.136 | 0.844 |
| 独立反射数 | 85799 | |
| <I/σ(I)> | 23.6 | 3.07 |
| 完全性 [%] | 100.0 | 100 |
| 冗長性 | 8.5 | 7.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 60% Tacsimate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
