3H2V

Human raver1 RRM1 domain in complex with human vinculin tail domain Vt

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-19
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 1
格子定数 [Å]	41.818, 70.921, 99.342
格子定数 [度]	89.77, 90.04, 104.04

精密化法

残基	20.000 - 2.900
R因子	0.2131
Rwork	0.210
R-free	0.27690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rke
結合長の平均二乗偏差(RMSD) [Å]	0.011
結合角の平均二乗偏差(RMSD) [度]	1.203
Refinement software	BUSTER-TNT (2.3.0)

Quality characteristics

	Overall
分解能 [Å] (低)	50.000
分解能 [Å] (高)	2.900
独立反射数	23190

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	100 mM Tris (pH 7.5), 200 mM sodium nitrate, and 16% PEG-3,350, VAPOR DIFFUSION, SITTING DROP, temperature 277K