3GQO
Crystal structure of macro domain of Venezuelan Equine Encephalitis virus in complex with ADP-ribose
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-29 |
| Detector | MAR555 FLAT PANEL |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 74.000, 87.200, 105.000 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 29.740 - 2.600 |
| R因子 | 0.199 |
| Rwork | 0.195 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gqe |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.433 |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.700 |
| 分解能 [Å] (高) | 2.600 | 2.600 |
| Rmerge_l_obs | 0.104 | 0.497 |
| 独立反射数 | 20549 | |
| <I/σ(I)> | 4.72 | |
| 完全性 [%] | 99.2 | 89.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 6.5 | 293 | 0.2M AmSO4, 0.1 M Na Cacodylate, 30 % PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
