3GBJ
Crystal structure of the motor domain of kinesin KIF13B bound with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97937 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.229, 86.520, 94.948 |
Unit cell angles | 90.00, 106.80, 90.00 |
Refinement procedure
Resolution | 39.062 - 2.102 |
R-factor | 0.222 |
Rwork | 0.221 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i6i |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.140 |
High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
Rmerge | 0.065 | 0.021 | 0.947 |
Number of reflections | 133690 | ||
<I/σ(I)> | 10.3 | ||
Completeness [%] | 99.9 | 98.7 | 100 |
Redundancy | 3.7 | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 18% PEG 3350, Ammonium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |