3FUY
Structure from the mobile metagenome of Cole Harbour Salt Marsh: Integron Cassette Protein HFX_CASS1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 32 |
| 格子定数 [Å] | 71.572, 71.572, 92.725 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 37.126 - 2.000 |
| R因子 | 0.1985 |
| Rwork | 0.195 |
| R-free | 0.23410 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.989 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 92.850 | 2.110 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.082 | 0.560 |
| Total number of observations | 202528 | |
| 独立反射数 | 35948 | |
| <I/σ(I)> | 15.3 | 4.2 |
| 完全性 [%] | 100.0 | 100 |
| 冗長性 | 5.6 | 5.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.2M ammonium sulfate, 25% PEG 3350 pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.2M ammonium sulfate, 25% PEG 3350 pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
