3FK1
E. coli EPSP synthase (TIPS mutation) liganded with S3P and glyphosate
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-11 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 57.886, 85.042, 87.690 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 20.000 - 1.700 |
| Rwork | 0.159 |
| R-free | 0.19000 |
| Starting model (for MR) | 1g6s |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.600 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 1.800 |
| 分解能 [Å] (高) | 1.700 | 1.700 |
| Rmerge_l_obs | 0.042 | 0.094 |
| 独立反射数 | 46727 | |
| <I/σ(I)> | 19.3 | 10 |
| 完全性 [%] | 96.7 | 91.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Na-formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
