3FEY
Crystal structure of the CBC-importin alpha complex.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.842, 104.505, 108.558 |
Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.200 |
R-factor | 0.19395 |
Rwork | 0.192 |
R-free | 0.23803 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1N54 and 1IAL |
RMSD bond length | 0.011 |
RMSD bond angle | 1.249 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.084 | 0.572 |
Number of reflections | 87239 | |
<I/σ(I)> | 12 | 2 |
Completeness [%] | 83.6 | |
Redundancy | 4.5 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 100 mM MES, pH 6.0, and 8% PEG 4000 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |