3ECR
Structure of human porphobilinogen deaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-12 |
| Wavelength(s) | 1.5798 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.610, 81.061, 109.211 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.276 - 2.182 |
| R-factor | 0.2337 |
| Rwork | 0.232 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.309 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.182 | 2.200 |
| Number of reflections | 29683 | |
| Completeness [%] | 88.9 | 55.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.15M ammonium citrate dibasic, 22%(w/v) PEG3350, pH8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
