3ECC

Crystal structure of the DnaC helicase loader in complex with ADP-BeF3

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-07
Detector	ADSC QUANTUM 210
Spacegroup name	P 61
格子定数 [Å]	84.582, 84.582, 49.587
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	50.000 - 2.700
R因子	0.263
Rwork	0.263
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	ELVES

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	73.000	2.230
分解能 [Å] (高)	2.120	2.120
独立反射数	5408
<I/σ(I)>	11.6	1.8
冗長性	3.9	2.7

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		8	294	50mM Tris, 1% PEG 6000, 1mM TCEP, 2mM ADP, 5mM MgCl2, 4mM BeCl2, 12mM NaF, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K