3CRT
Structural characterization of an engineered allosteric protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2007-06-05 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 91.180, 91.180, 57.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.500 - 1.900 |
Rwork | 0.227 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBID: 1GNE |
RMSD bond length | 0.007 |
RMSD bond angle | 1.408 |
Data reduction software | PROTEUM PLUS (2) |
Data scaling software | PROTEUM PLUS (2) |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.500 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.067 |
Number of reflections | 34548 |
<I/σ(I)> | 20.56 |
Completeness [%] | 94.6 |
Redundancy | 9.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG8000, 50mM tris, 5mM B-mercaptoethanol , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |