3CRO
THE PHAGE 434 CRO/OR1 COMPLEX AT 2.5 ANGSTROMS RESOLUTION
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Detector | XENTRONICS |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 49.200, 47.600, 61.700 |
| 格子定数 [度] | 90.00, 109.50, 90.00 |
精密化法
| 残基 | 10.000 - 2.500 |
| R因子 | 0.22 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.300 |
| Refinement software | TNT |
Quality characteristics
| Overall | |
| 分解能 [Å] (高) | 2.500 |
| Rmerge_l_obs | 0.101 * |
| 独立反射数 | 7232 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | unknown * | 6.2 | 4 * | pH 6.20, temperature 277.00K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 3500 | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 1 | SPERMINE |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | 2 | PEG3500 | 12 (%(w/v)) | |
| 2 | 1 | 2 | MES | 100 (mM) | pH6.2 |
| 3 | 1 | 2 | 160 (mM) | ||
| 4 | 1 | 2 | 120 (mM) | ||
| 5 | 1 | 2 | spermine | 2 (mM) | |
| 6 | 1 | protein | 2 (mM) | ||
| 7 | 1 | 1 | DNA | 1 (mM) |
