3C7R
Crystal Structure of HIV-1 subtype F DIS extended duplex RNA bound to neomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2006-11-19 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.130, 30.330, 58.330 |
Unit cell angles | 90.00, 120.79, 90.00 |
Refinement procedure
Resolution | 29.070 - 1.700 |
R-factor | 0.251 |
Rwork | 0.251 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c3z |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.070 | 1.750 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 31607 | |
<I/σ(I)> | 43.2 | 3.3 |
Completeness [%] | 97.5 | 79.1 |
Redundancy | 8.5 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 310 | MPD, KCl, MgCl2, Na cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Na cacodylate | ||
2 | 1 | 1 | MgCl2 | ||
3 | 1 | 1 | KCl | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 2 | Na cacodylate | ||
6 | 1 | 2 | MgCl2 | ||
7 | 1 | 2 | KCl | ||
8 | 1 | 2 | MPD |