3BCC
STIGMATELLIN AND ANTIMYCIN BOUND CYTOCHROME BC1 COMPLEX FROM CHICKEN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-29 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 173.180, 179.730, 238.220 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 12.000 - 3.700 |
| R因子 | 0.289 |
| Rwork | 0.289 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT USING NATIVE STRUCTURE SOLVED BY THE SAME AUTHOR |
| Starting model (for MR) | 1bcc |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 21.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TFFC |
| Refinement software | CNS (0.1) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 3.630 | |
| 分解能 [Å] (高) | 3.500 | 3.500 |
| Rmerge_l_obs | 0.156 | 0.383 |
| 独立反射数 | 80888 | |
| <I/σ(I)> | 6.6 | 1.6 |
| 完全性 [%] | 85.8 | 75.7 |
| 冗長性 | 2.84 | 1.38 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion * | 6.7 | 20MM KMES PH6.7, 75MM NACL, 10% GLYCEROL, AND 6% PEG4000, INHIBITOR WAS ADDED FROM ETHANOLIC SOLUTION |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | KMES | 60 (mM) | pH6.7 |
| 2 | 1 | drop | PEG4000 | 8 (%) | |
| 3 | 1 | drop | K-MOPS 7.5 | 20 (mM) | |
| 4 | 1 | drop | n-octyl-beta-D-glucopyranoside | 20 (g/l) | |
| 5 | 1 | drop | 87.5 (mM) | ||
| 6 | 1 | drop | glycerol | 10 (%) | |
| 7 | 1 | reservoir | glycerol | 30 (%) |
