3AXI
Crystal structure of isomaltase in complex with maltose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.474, 115.373, 61.594 |
Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
Resolution | 18.830 - 1.400 |
R-factor | 0.17991 |
Rwork | 0.179 |
R-free | 0.19432 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3aj7 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.427 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.830 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.062 | 0.120 |
Number of reflections | 121866 | |
<I/σ(I)> | 18 | 10.5 |
Completeness [%] | 92.9 | 94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 288 | 50mM HEPES, 0.2M lithium acetate, 21% (w/v) PEG 3350, 0.2M maltose , pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 288K |