3AKA
Structural basis for prokaryotic calcium-mediated regulation by a Streptomyces coelicolor calcium-binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-07-31 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.876, 51.044, 86.998 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.220 - 1.800 |
R-factor | 0.19926 |
Rwork | 0.197 |
R-free | 0.24826 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Se-Met structure of CabD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.336 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.054 | 0.337 |
Number of reflections | 21409 | |
<I/σ(I)> | 11.9 | 2.8 |
Completeness [%] | 98.9 | 94.6 |
Redundancy | 4.3 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 8000, Cacodylate Sodium, Zinc Chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |