3AIZ
Crystal structure of PCNA2-PCNA3 complex from Sulfolobus tokodaii (P21212)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-26 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 91.110, 160.570, 116.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.640 - 2.800 |
R-factor | 0.2248 |
Rwork | 0.223 |
R-free | 0.25630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3aix |
RMSD bond length | 0.002 |
RMSD bond angle | 0.472 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.095 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 42314 | |
<I/σ(I)> | 10.3 | 4.5 |
Completeness [%] | 98.9 | 97.6 |
Redundancy | 7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.6M ammonium sulfate, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |