3AIX
Crystal structure of PCNA2-PCNA3 complex from Sulfolobus tokodaii (I222)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| 格子定数 [Å] | 91.100, 111.800, 170.900 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 48.301 - 2.900 |
| R因子 | 0.2123 |
| Rwork | 0.210 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2izo |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.002 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.470 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.5_2) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 93.549 | 3.060 |
| 分解能 [Å] (高) | 2.900 | 2.900 |
| 独立反射数 | 19736 | |
| <I/σ(I)> | 19.7 | 5.4 |
| 完全性 [%] | 100.0 | 100 |
| 冗長性 | 10.7 | 10.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.6M ammonium sulfate, 0.1M sodium Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
