3A3Q

Structure of N59D HEN EGG-WHITE LYSOZYME in complex with (GlcNAc)3

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	281
Detector technology	IMAGE PLATE
Collection date	1995-06-19
Detector	MAC Science DIP-320
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
格子定数 [Å]	78.710, 78.710, 38.610
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	50.000 - 2.000
R因子	0.21284
Rwork	0.205
R-free	0.28656
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lza
結合長の平均二乗偏差(RMSD) [Å]	0.011
結合角の平均二乗偏差(RMSD) [度]	1.339
Data reduction software	PROTEIN
Data scaling software	PROTEIN
Phasing software	REFMAC (5.5.0088)
Refinement software	REFMAC (5.5.0088)

Quality characteristics

	Overall
分解能 [Å] (低)	50.000
分解能 [Å] (高)	2.000
Rmerge_l_obs	0.098
独立反射数	6611
完全性 [%]	76.6
冗長性	3.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	4.7	293	10% sodium chloride, 0.1M sodium acetate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K