3A2G
Crystal Structure of K102C-Myoglobin conjugated with Fluorescein
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-12 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 6 |
| 格子定数 [Å] | 90.409, 90.409, 45.320 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 22.610 - 1.750 |
| R因子 | 0.17869 |
| Rwork | 0.178 |
| R-free | 0.19746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mbw |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.348 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 1.810 |
| 分解能 [Å] (高) | 1.750 | 1.750 |
| Rmerge_l_obs | 0.092 | 0.304 |
| 独立反射数 | 21588 | |
| <I/σ(I)> | 30 | 5.4 |
| 完全性 [%] | 100.0 | 99.9 |
| 冗長性 | 5.5 | 5.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 2.6M ammonium sulfate in 20mM Tris-HCl, pH9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
